Picogreen assays are compatible with most microplate readers and fluorometers. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Catalog Number. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. Picogreen assays are compatible with most microplate readers and fluorometers. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. VIEWS. a) 16. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. PicoGreen®試薬アッセイ. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. 参考資料. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. 8. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). BR reagent (Component A) 1. 2–400ng/µl (1µl sample in 200µl assay). Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. P7589. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. Three kits covering a wide range of dsDNA concentrations. Quant-iT™ PicoGreen ® dsDNA Reagent (10 vials, each containing 100 µL aliquots) 25 mL of 20X TE buffer; 1 mL of 100 µg/mL Lambda DNA (dsDNA standard) These jellies cheapest cialis uk and pills are can be ingested with or without food. 99 to $1999. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. Barcoded amplicons were pooled in. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. quant it picogreen dsdna assay kit , 2023-10. Reagent Search & Data Services Menu Reagent Search. 5 µL of each extracted sample was used for Picogreen analysis. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The Quant-iT™ DNA Assays are designed for high throughput (>20 samples) and for use in 96-well plate readers with no further adaptation. 326,933 327K. The PicoGreen is sticking to the sides of the cuvette or assay plate. The dynamic range of this assay in microplate format, as stated in the product manual, is from 250 pg/mL to 1000 ng/mL with a single dye concentration. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. Picogreen assays are compatible with most microplate readers and fluorometers. The Quant-iTTM PicoGreen® dsDNA Reagent is an ultra-sensitive fluorescent nucleic acid stain for quantitating double-stranded DNA (dsDNA) in molecular biology procedures. The fusion protein nsp7-nsp8 was generated by inserting a GSGSGS linker sequence. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Picogreen assays are compatible with most microplate readers and fluorometers. Our Quant-iT™ PicoGreen® dsDNA reagent enables researchers to quantitate as little as 25 pg/mL of dsDNA (50 pg dsDNA in a 2 mL assay volume) with a standard spectro fluoro- meter and. Both fluorescent assays were performed with a Qubit fluorometer. • Suitable for use in 96- and 384-well plate formats. Each sample was amplified in triplicate, and the triplicate amplicons were pooled and purified by gel extraction and quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, P7589). Quant-iT PicoGreen dsDNA Assay Kit (1mL) Quant-iT PicoGreen dsDNA Reagent (1mL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P7589 P7581: Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Fluorescence signal measured for the DNA sodium salt standard from calf thymus using the Quant-iT™ PicoGreen® assay. For duplicate-well droplet. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. 2 DNA selectivity and sensitivity of the Quant-iT dsDNA high-sensitivity assay. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. Bioz Stars score: 86/100, based on 1 PubMed citations. 50 ul PicoGreen Mix by swirling bottle. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. dsDNA Assay Kit: Invitrogen Quant-iT PicoGreen dsDNA Assay Kits and dsDNA Reagents, spectrophotometer, qPCR Quantification Kit: Roche KAPA Library Quantification Kits — Complete Kit (Universal), and qPCR Plate Reader: LightCycler 480 Instrument II. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. 2. 2. • Suitable for use in 96- and 384-well plate formats. (D–G) TLR stimulation was measured using a HEK293-TLR reporter. 1 ng/μL to 2000 ng/μL, with an extended upper range to 4000 ng/μL. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. The fluorescence intensity of PicoGreen was measured to determine the. Prepare the 200 fold dilution of pico dye to be kept in the dark. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Rapidly detect as little as 0. Bioz Stars score: 86/100, based on 1 PubMed citations. 3,201 3. (Center) The Quant-iT RiboGreen RNA Assay provides linear quantitation. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Signal measurements of all samples were performed using the EnVision 2105 Multimode Plate Reader (PerkinElmer, Waltham, MA,. 95 mL TE for the . 适用于（设备）. 522. Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. • Specific for dsDNA in the presence of RNA. • Specific for dsDNA in the presence of RNA. The The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. The kit is robust with a mix and read format compatible with 96- and 384-well fluorescence-based microplate readers. Perform dsDNA quantification easily and quickly with Quant-iT dsDNA assay kits. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. The Quant-iT™ PicoGreen® dsDNA reagent is a proprietary, asymmetrical cyanine dye. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). 10 x 100 µL. Technical Support Customer Service. Quantitation Range. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). Add 5uL of PicoGreen reagent for every 1mL of buffer. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. PicoGreen dsDNA quantitation reagent is a highly sensitive fluorescent nucleic acid stain which is used to quantitate double-stranded DNA. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). In addition, the concentration of DNA obtained from the 1860 tomato samples was measured fluorometrically using Quant-iT™ PicoGreen™ dsDNA Assay Kit (hereafter PicoGreen, Thermo Scientific, Waltham, MA, USA) and a 96-wells plate reader VICTOR3 1420 (PerkinElmer, Waltham, MA, USA) equipped with an excitation filter F485 and. 5ul of 12uM primer diluted in ClontechPicoGreen is highly linear (R 2 ≥ 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. PCR products were quantified using the Quant-iT PicoGreen dsDNA Assay Kit on a Microplate reader (Bio-Tek, FLx800), and then mixed according to the amount of data required for each sample. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for each cell line of interest. This sensitivity exceeds that achieved with the Hoechst 33258. 3 Q33120 39. After the reagent thawed, it is advisable to briefly vortex the tube toPlasma levels of cfDNA were measured using a fluorometric assay for double-stranded DNA Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) following the manufacturer’s instructions. Figure 2. The Quant-iT RNA Reagent and Assay Kit makes RNA quantification easy and accurate. Quant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. Picogreen assays are compatible with most microplate readers and fluorometers. 99. Picogreen assays are compatible with most microplate readers and fluorometers. 微孔板读数仪. The final pooled sample was quantified using a Quant-IT PicoGreen dsDNA assay (Thermo Fisher Scientific) and the resulting fragment size distribution was assessed using a Bioanalyzer (Agilent. ZERO BIAS - scores, article reviews, protocol conditions and moreComparison of dsDNA dose-response using the Helixyte Green™ Fluorimetric dsDNA Quantitation Kit *High Sensitivity* (blue) with Quant-iT™ PicoGreen ® dsDNA Assay Kit (red ). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). ZERO BIAS - scores, article reviews, protocol conditions and morePicogreen assay with Qubit platform is considered to be the method of choice for DNA quantification. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). • Suitable for use in 96- and 384-well plate formats. Q33130). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. This is Standard 1 which will have a final concentration of 1 ug/mL. Quant-iT PicoGreen dsDNA Assay Kit a. 2–100 ng, the fluorescence signal is linear with DNA. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Optimized for fluorescence microplate readers. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). The total amount of DNA expected is in the range of 3–100 ng, with concentrations as low as 0. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Quant-iT RiboGreen RNA Reagent The Quant-iT RiboGreen RNA Reagent is one of the most sensitive fluorescent dyes for the quantitation of RNA in solution, with linear fluorescence detection in the range of 1-200 ng of RNA. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Rapidly detect as little as 0. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. o cDNA quantification using Quant-iT PicoGreen dsDNA Assay Kit (P7589, Life Technologies) o PCR Cycles used as indicated in NUM_PCR_CYCLES_cDNA and NUM_PCR_CYCLES_Nextera x C1_ADD_Reagents: o Randomer added to Lysis Reaction Mix, (3. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. Regulations & Guidance; Manuals 0 0. Rapidly detect as little as 0. no. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. Quantitation Range. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. RESULTS The OT-2 Constructs Highly Uniform LibrariesThermo Fisher double stranded dna quant it picogreen dsdna assay kit Double Stranded Dna Quant It Picogreen Dsdna Assay Kit, supplied by Thermo Fisher, used in various techniques. Figure 8. Quant-iT dsDNA BR buffer (Component B) 250 ml c. The kit included 20X TE buffer (200 mM Tris-HCl, 20 mM EDTA, pH 7. Plasma levels. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Volume of 20xTE = final volume/20;Application tip demonstrating that with Molecular Devices SpectraMax® microplate textbooks and the Quant-iT PicoGreen assay, users can reliably measure contents as low when 50 pg/mL of double-stranded DNA. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. methods for the quantitation of DNA: the Invitrogen™ Quant-iT™ PicoGreen™ dsDNA assay and the Invitrogen™ Qubit™ dsDNA assays. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). The DNA amount in each library was quantified with the Quant-iT PicoGreen dsDNA Assay Kit and with the Agilent 2200 TapeStation. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Description. According to the obtained results, the assay presents a linear correlation in a wide range of 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The Quant-iTTM dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. Table 2 summarizes results of the. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Revised: 10–June–2008 | MP 07581 Table 1. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 7. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Rapidly detect as little as 0. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT PicoGreen dsDNA. 3. Description. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. The assay is performed at room temperature, and the signal is stable for 3 hours. The Molecular Probes PicoGreen®. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. 0 ml 200x in DMSO b. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. One microgram of DNA was sheared using an M220. 激发/发射. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Picogreen assays are compatible with most microplate readers and fluorometers. Cloning, expression and proteins purification. Quantitation Range. Product Type. Using this data as a starting point, the optimal conditions (e. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. DNA was normalized to 2 concentrations, 100+/-20µg/mL and 20+/-5µg/mL. The assay is linear over three orders of magnitude and has little sequence dependence. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. Bioz Stars score: 99/100, based on 1 PubMed citations. This protocol is a simplified and condensed version of. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Selectively detect dsDNA from a wide variety of sources, and even in presence of. DNA standard curve. 10 x 100 µL. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. 1 Active esters and kits for labeling proteins and. Quant-iT RNA BR (Broad Range) Assay Kit. Mit dem PicoGreen dsDNA. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. high-range. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. 10 ng/mkl – 10000 ng/ml. Unit Size. The assay is a homogeneous, mix-and-read format where the fluorescence is proportional to analyte present. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. Q33130). 2–100 ng, the fluorescence signal is linear with DNA. Using this data as a starting point, the optimal conditions (e. Free dye. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Description. Unit Size. Quantitation Range. samples as well as standard in the assay itself. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. In case dsDNA standards are available only the quantification reagent can be used. 7 Q33231 6 1,000 41. 2 ng to 100 ng of DNA in the assay tube. 文件. The original sequencing data. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Rapidly detect as little as 0. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. Picogreen assays are compatible with most microplate readers and fluorometers. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Fluorescence was measured at 485/530 nm and plotted versus the mass of nucleic acid for the DNA alone,. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. The assay is highly selective for double- stranded DNA over RNA, and in the. • Specific for dsDNA in the presence of RNA. The reagent must be completely thawed before using it by bringing it to room temperature. Picogreen assays are compatible with most microplate readers and fluorometers. Calibrator MPO concentration was quantified with a commercially available assay (Human Myeloperoxidase Quantikine ELISA Kit, Bio-Techne, Minneapolis, MN) according to the manufacturer’s recommendations. BR reagent (Component A) 1. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. 作成者 LatB Staff 01. No. Samples were excited at 480 nm and the fluorescence was measured at 520 nm using a TECAN Spark multimode reader. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. Highly selective for dsDNA over ssDNA or RNA. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. no. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For a standard. The range of linearity for DNA concentration measurements. Qubit DNA and RNA assays are available with different quantification ranges. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. The linear detection range of the PicoGreen® assay in the 20/20n extends over three orders of magnitude in DNA concentration - from 450 pg/mLFor DNA quantification with PicoGreen (PG), Quant-iT™ PicoGreen® dsDNA Reagents and Kits (Invitrogen – Molecular Probes, Eugene, OR, USA) were used. PicoGreen dsDNA Assay Kit. 50 pg to 2 µg. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Quant-iTTM PicoGreen® Assay Reagent 0. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. Price from $9. i) 110 sample x 150uL =16. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. 99. Bioz Stars score: 86/100, based on 1 PubMed citations. Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. Quantitation Range. For running these libraries on the MiSeq and HiSeq, please make sure you read the supplementary methods of Caporaso et al. The kit contains. PicoGreen dsDNA quantitation reagents are. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. Quant-iT PicoGreen Reagent Final DNA Concentration in Quant-iT PicoGreen Assay 0 1000 25 ng/mL 900 100 1000 2. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. The method for this assay follows the instructions in the product information sheet for Quant-iT PicoGreen dsDNA Reagent and Kits from Molecular Probes, except that the assay volume is proportionately reduced from 2. Silke Angersbach, Ron Earp, Mark Gröne, Franka Maurer, BMG LABTECH, 12/2014. Catalog Number. Depending on sample volume, the assay kit is designed to be accurate for initial DNA sample concentrations of 0. 0 fluorometer and Quant-iT PicoGreen dsDNA. It is specific for dsDNA in the presence of equimolar amounts of RNA. Similar. Equal amounts (100 µL) of working PicoGreen quantitation reagent were mixed and incubated for 10 minutes at room temperature, protected from light. 2. DNA standard curve. • Suitable for use in 96- and 384-well plate formats. • Suitable for use in 96- and 384-well plate formats. Product Type. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. 5), 200X PicoGreen. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). Thermo Fisher quant it picogreen dsdna assay kit. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. Quant-iT PicoGreen dsDNA Reagent. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 2. • Suitable for use in 96- and 384-well plate formats. Quantification and normalization of double stranded DNA with minimal set-up time. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R114909. We use the Quant-iT™ PicoGreen® dsDNAkit for quantification of both extracted and amplified DNA. 2. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Breadcrumb. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. Catalog Number. 4 mL of prewarmed to 37°C, TE Buffer Cat# D001 or other qualified TE buffer. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 5 – 1,000 ng/ mL DNA with a high dynamic range (Fig. Buy from Supplier. , QuantIt PicoGreen dsDNA assay). Picogreen assays are compatible with most microplate readers and fluorometers. with the Qubit dsDNA BR Assay Kit and 1 µL of each sample was measured in quadruplicate with the Quant-iT™ PicoGreen dsDNA Assay Kit. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Rapidly detect as little as 0. Figure 2. Picogreen assays are compatible with most microplate readers and fluorometers. We recommend using Quant-IT Picogreen as a high-sensitivity assay to measure DNA concentration in that range . 5 ng/mkl – 100 mg/ml. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). 作成者 LatB Staff 01. assay. Skip to main list 美谷分子仪器（上海）有限公司. For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2. Price from $9. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. Mix by gently vortexing for 5 seconds. Abstract. A 96 plate well was filled with 100 µl of dye and 100 µl of DNA, a 384 well with 10 µl of each solution. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double. 10. 0 fluorometer—provides state-of-the-art reagents for sensitive and selective quantitation of DNA, RNA or protein samples using a standard fluorescence microplate reader or the Qubit 2. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. 22. Tweet. Dilute from 20xTE stock (supplied in the PicoGreen kit). After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. PicoGreen Solution must be diluted fresh before each assay run. 2–100 ng of DNA. PicoGreen dsDNA quantitation reagents are. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). Figure 8. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Qubit™ dsDNA Quantification Assay Kits. Bioz Stars score: 86/100, based on 1 PubMed citations. For duplicate-well droplet. The libraries were sequenced using the Illumina HiSeq 2500. Figure 1. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use.